Plant/Algal cell antibodies / Compartment markers / Cytoplasm

product information

Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311)  is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase

overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase Q9MA79

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

collection of other antibodies which can be used as markers of cytoplasm

Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector.

application information

1: 5 000 with standard ECL (WB)

45 | 37 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Brassica napus, Pinus silvestris, Oryza sativa , Zea mays cellular compartment marker of cytoplasm in photosynthetic tissues

dicots including Pisum sativum, Solanum tuberosum and monocots including Oryza sativa, Zea mays

no confirmed exceptions from predicted reactivity known in the moment

for image of detection please refer to Strand et al. 2000. This antibody does not react with chloroplastic form of FBPase.

Hayashi et al. (2011). Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the ER stress response of rice. Plant J. Nov

Strand et al. (2000). Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences forphotosynthetic carbon metabolism in transgenic Arabidopsis thaliana.  The Plant Journal,  23(6), 759-770.