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product information
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| background |
|
In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. |
| immunogen |
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His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23) |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
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100 µl |
for reconstitution add 100 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), immunoprecipitation (IP) |
| related products |
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Collection of antibodies to Chlamydomonas proteins |
| additional information |
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NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii. |
application information
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| recommended dilution |
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1: 10 000 standard ECL (WB) |
| expected | apparent MW |
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26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii) |
| confirmed reactivity |
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Chlamydomonas reinhardtii |
| predicted reactivity |
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Chlamydomonas reinhardtii |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Blifernez et al. (2011) Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. Plant J. 65: 119-130. Wobbe et al. (2009) Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proc. Natl. Acad. Sci. USA 106: 13290-13295 Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421 |
application example
Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1. | 
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