In Chlamydomonas reinhardtiiNAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis.
immunogen
His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)
antibody format
rabbit
polyclonal
serum
lyophilized
quantity
100 µl
for reconstitution add 100 µl of sterile water.
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.
application information
recommended dilution
1: 10 000 standard ECL (WB)
expected | apparent MW
26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)
confirmed reactivity
Chlamydomonas reinhardtii
predicted reactivity
Chlamydomonas reinhardtii
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
to be added when available
selected references
Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421
application example
Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.
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