Plant/Algal cell biology / Compartment markers / Cytoplasm

product information

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

store lyophilized/reconstituted at -20�°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS03 035A   SPS | sucrose phosphate synthase, global

for other antibodies to various maize proteins, please inquire.

total IgG concentration is 3 µg/µl

application information

1 : 2 000 with standard ECL (WB)

120 | ~130 for Zea mays

Zea mays

Zea mays and the monocots Saccharum officinarum, Triticum aestivum and Oryza sativa

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Sparks et al. (2001) A Appl Biol 138: 33-45

application example

10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).