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product information
|
| background |
|
Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex. |
| immunogen |
|
purified SHMT protein from Spinacia oleracea |
| antibody format |
|
rabbit |
polyclonal |
|
total IgG in PBS pH 7.4 |
lyophilized |
|
| quantity |
|
200 µl |
for reconstitution add 200 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS05 074 | anti-GDC-H rabbit antibody, marker of mitochondrial matrix AS06 203A | anti- Idh | isocytrate dehydrogenase rabbit antibody, marker of mitochondrial matrix AS07 212 | anti-VDAC1 marker antibody for mitochondrial outer membrane |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
|
1:5 000 with standard ECL (WB) |
| expected | apparent MW |
|
53 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
|
Arabidopsis thaliana, Spinacia oleracea |
| predicted reactivity |
|
dicots including: Glycine max, Medicago truncatula, Pisum sativum, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
this antibody can be used on total leaf extracts and isolated mitochondria |
| selected references |
|
to be added when available |
application example
total leaf extract from Arabidopsis thaliana has been loaded per lane based on a leaf disc area. Primary antibody has been used in dilution 1: 5000 following detection using standard ECL. c
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||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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