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product information
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| background |
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BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). |
| immunogen |
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KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 protein. |
| antibody format |
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rabbit |
polyclonal, |
affinity purified serum |
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| quantity |
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200 µg |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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ELISA (ELISA), western blot (WB), immunofluorescence (IF), immunogold (IG) |
| related products |
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AS09 615 | BiP2 | lumenal-binding protein 2, goat antibody AS09 614 | BiP2 | lumenal-binding protein 2, hen antibody antibodies to plant endomembrane system proteins |
| additional information |
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Method for plant ER isolation is available here. |
application information
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| recommended dilution |
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1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 600 ( IF) |
| expected | apparent MW |
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73.5 | 80 kDa |
| confirmed reactivity |
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dicots: A. thaliana, B. napus, C. sativus, R. sativa L. Tokinashi-daikon, S. oleracea, S. lycopersicum, S. tuberosum, monocots: Z. mays, moss: P. patens, algae: Ch. reinhardtii |
| predicted reactivity |
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dicots including: Nicotiana tabacum, monocots: Hordeum vulgare, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. |
| selected references |
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Wulfetange et al. (2011). The Cytokiin Receptors of Arabidopsis thaliana are Locating Mainly to the Endoplasmic Reticulum. Plant Physiol. (in press). |
application example western blot
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5 µg of total protein from A.thaliana (1), H. vulgare (2), P. sativum (3)*, Z. mays (4), C. sativus(5), S. tuberosum (6), S. oleracea (7), S. lycopersicum (8) P. patens (9)*, Ch. reinhardtii (10) extracted with Agrisera PEB extraction buffer (AS08 300) were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. * Lack of the signal or its low signal intensity in those samples can be due to the sample biology. If you work with those species, please inquire. application example immunolocalization  BiP localization in 5 days old Arabidopsis thaliana roots (A), 3 days old Triticum aestivum roots (B).
BiP signal shown in red, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-BiP primary antibody diluted in 1: 600 and ALEXA 555 conjugated anti-rabbit secondary antibody (red color) have been used. Co-staining with DAPI visualized nucleus (blue color). Scale bar – 10 µm. Courtesy Dr. Taras Pasternak, Freiburg University
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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