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product information
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| background |
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SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutté et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD |
| immunogen |
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KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02 |
| antibody format |
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rabbit |
polyclonal |
|
affinity purified IgG |
lyophilized |
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| quantity |
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200 µg, |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunolocalization (IL) |
| related products |
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AS09 481 | anti-BiP2 | luminal-binding protein 2 antibodies to other proteins of endomembrane system |
| additional information |
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SMT1 is an integral membrane protein of ER (Boutté et al., 2009) while BiP is a membrane associated protein. |
application information
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| recommended dilution |
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1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL) |
| expected | apparent MW |
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38 kDa |
| confirmed reactivity |
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Arabidopsis thaliana
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| predicted reactivity |
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dicots including: Glycine max, Gossypium mexicanum, Ricinus communis
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| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Boutté Y, Rosa M.S.F, Men S, Chow C.M, Ebine K, Gustavsson G, Johansson L, Ueda T, Moore I, Jürgens G, Grebe M (2009). Endocytosis Restricts Arabidopsis KNOLLE Syntaxin To The Cell Division Plane During Late Cytokinesis. EMBO J open access
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||| For applications or usage on species others than stated above as confirmed Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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