Plant/Algal cell antibodies / Compartment markers / Mitochondrial marker

product information

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including  Oryza sativa P04373 and Physcomitrella patens Q1XGA9

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), Blue Native-PAGE (BN-PAGE)

AS04 052 anti-COXII hen antibody

AS04 151 ant-COXIIb rabbit antibody

antibodies to other mitochondrial proteins

recommended secondary antibody

cellular [compartment marker] of mitochondrial inner membrane

application information

antibody detects COXII protein in total leaf extracts and isolated mitochondria

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)

Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,
salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.

Pertl et al. (2009).  The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen. j. Proteome Res. 11:5142.5152.

Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.

application example

1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.