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product information
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| background |
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VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004). |
| immunogen |
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KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280 |
| antibody format |
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rabbit |
polyclonal |
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affinity purified serum, in PBS pH 7.4 |
lyophilized |
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| quantity |
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200 µg |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), Blue-native (2D BN/SDS-PAGE), immunolocalization (IL) |
| related products |
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AS04 054 | anti-AOX1/2 rabbit antibody, marker of mitochondrial inner membrane AS06 203A | anti-Idh rabbit antibody, marker of mitochondrial matrix collection of antibodies to other mitochondrial proteins |
| additional information |
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cellular [compartment marker] of mitochondrial outer membrane |
application information
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| recommended dilution |
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1:5000 on 2-30 µg of protein/lane with standard ECL (WB), 1: 500 (IL) |
| expected | apparent MW |
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29 kDa (for Arabidopsis thaliana) |
| confirmed reactivity |
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Arabidopsis thaliana, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrella patens, |
| predicted reactivity |
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dicots including Nicotiana tabacum, Plantago major, Ricinus communis,monocots including Triticum aestivum,
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| not reactive in |
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Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms
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| additional information |
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Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg. Immunolocalization method description and images are available here Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010 |
| selected references |
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Juszczuk and Ostaszewska (2011). Respiratory activity, energy and redox status in sulphur-deficient bean plants. Environm and Exper Botany in print. Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source) Gibala et al. (2009). The lack of mitochondrial AtFtsH4 protease alters Arabidopsis leaf morphology at the late stage of rosette development under short day photoperiod. Plant J.In press. |
application example
10 μg of mitochondrial fraction from Arabidopsis thaliana and 25 μg of Arabidopsis thaliana leaf extract were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 5000 anti-VDAC1 antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7). Courtesy Dr. Janusz Piechota, Wrocław University, Poland
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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