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product information
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| background |
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Hydroxypyruvate reductase (HPR) belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family (oxidoreductases) and is involved in glycine, serine and theronine and glyoxylate and dicarboxylate metabolism. Synonymes: HPR, beta-hydroxypyruvate reductase, NADH:hydroxypyruvate reductase, D-glycerate dehydrogenase. |
| immunogen |
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KLH-conjugated synthetic peptide derived from known plant HRP sequences, including Arabidopsis thaliana Q9C9W5 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
|
| quantity |
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100 µl |
for reconstitution add 100 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS08 372 | AtPex14p | peroxysomal marker, rabbit antibody AS10 711 | DEG15 | endopeptidase, peroxisomal marker, rabbit antibody |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 10 000 with standard ECL (WB) |
| expected | apparent MW |
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43 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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dicots including: Cucumis sativus, Glycine max, Ricinus communis, monocots inlcuding: Oryza sativa, Chlamydomonas reinhardtii, Volvox, Chlorella sp. |
| not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in November 2011. |
application example 
5-day-old light-grown wild-type (Columbia) (1) and hpr1-1 null mutant (SALK_143584) Arabidopsis thaliana seedlings were ground with a pestle in a 1.5 mL tube on dry ice (about 12 seedlings or enough to give ~ 20 µL of tissue), and double volume (~ 40 µL) of NuPAGE 2x loading buffer (Invitrogen) was added. After centrifugation, 20 µL of the supernatant was transferred to a fresh tube with 2.1 µL 0.5 M DTT and boiled at 100 °C for 5 minutes. Samples were loaded onto a NuPAGE 10% Bis-Tris gel (Invitrogen) next to Cruz Markers (Santa Cruz Biotechnology). After electrophoresis, proteins were transferred for 30 minutes at 24 V to a Hybond ECL nitrocellulose membrane (Amersham Pharmacia Biotech) using NuPAGE transfer buffer (Invitrogen). The blot was blocked for 1 h at 4 °C in 8% non-fat dry milk in TBS-T (blocking buffer), and incubated overnight with agitation at 4˚C with primary antibodies, 1:10 000 diluted in blocking buffer. The antibody solution was decanted and the blot was rinsed twice for 5 min each at 4 °C in 8% non-fat dry milk in TBS-T with agitation. The blot was incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Santa Cruz Biotechnology) diluted to 1:5 000 in blocking buffer for 5 h at 4 °C with agitation. The blot was washed three times, for 5 min each, with TBS-T and developed with LumiGLO reagent (Cell Signaling Technology) according to the manufacturer's instructions. Exposure time was 3 seconds. Courtesy of Sarah Bukhart and Bonnie Bartel, Rice University, USA
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