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product information
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| background |
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Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions. |
| immunogen |
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BSA-conjugated abscisic acid |
| antibody format |
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rabbit |
polyclonal |
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total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35 |
lyophilized |
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| quantity |
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100 µl, |
for reconstitution add 50 µl, of sterile water and 50 µl of glycerol |
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| storage |
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store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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ELISA (ELISA), immunolocalization (IL) |
| related products |
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AS09 446 | ABA | abscisic acid (C1) (for immunolocalization) AS09 422 | ABA | abscisic acid (C1) (quantity 1mg) AS09 447 |anti-ABA | abscisic acid (C1) (quantity 5mg) collection of antibodies to other plant hormones |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5000 - 1: 10 000 (ELISA), 1: 100 (IL) |
| expected | apparent MW |
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n.a. |
| confirmed reactivity |
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Euphorbia pulcherrima |
| predicted reactivity |
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dicots and monocots |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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for detailed immunolocalization protocol check "more information" |
| selected references |
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to be added when available |
application example 
ABA localization in 5 days old roots of Arabidopsis thaliana. ABA signal is shown in red, PIN2 signal shown in green, DAPI in blue. The material has been fixed with 4 % EDAC for 10 min followed by EDAC and para-formaldehyde. Tissue cleaning has been performed before immunolocalization. Rabbit anti-ABA primary antibody diluted in 1: 400, ALEXA 555 conjugated anti-rabbit secondary antibody, diluted 1: 700 (Invitrogen). Co-staining with DAPI visualized nucleus (blue color). Courtesy Dr. Taras Pasternak, Freiburg University, Germany
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