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product information
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| background |
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Goat anti-rabbit IgG, DyLight® 488 Conjugate is a secondary antibody conjugated to DyLight® 488, which binds to all rabbit IgGs in immunological assays. The dye has a green color and absorbs at 493 nm and emits at 518 nm. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. |
| antibody format |
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goat |
polyclonal |
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purified goat IgG |
lyophilized |
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| quantity |
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1 mg |
for reconstitution add 1,1 ml of sterile water. |
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| storage |
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Store undiluted liquid at 2-8°C. Shelf life is one year from a date of receipt. |
| related products |
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other anti-rabbit DyLight® conjugated secondary antibodies |
| recommended dilution |
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1:20 – 1:2 000 (IHC), 1:50 – 1:5 000 (ICC), 1: 3000 (IF) |
| additional information |
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Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm
Fluor to protein ratio is: 1-5 Moles DyLight® 488 per Mole Antibody
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
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 BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, PIN2 in red , DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibody Agrisera anti-rabbit BiP (AS09 481) diluted in 1: 600. Secondary antibodies: green color Agrisera DyLight® 488 goat anti-rabbit (AS09 633) diluted in 1: 3000, red color anti-GP Alexa 555 (Invitrogen), 1: 600. Courtesy Dr. Taras Pasternak, Freiburg University
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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