Plant/Algal cell antibodies / Conifers

product information

GSNO; S-nitrosoglutathione belongs to a family of molecules involved in nitrosative stress. GSNO is a natural source of nitric oxide (NO). S-nitrosylation reaction of NO with GSH leads to formation of S-nitroso glutathione (GSNO). Selected references: Barroso et al (2006). Localization of S-nitrosoglutathione and expression of S-nitrosoglutatione reductase in pea plants under cadmium stress.  J Exp Bot. 2006;57(8):1785-93. Valderrama et al (2007). Nitrosative stress in plants. FEBS Lett. 6;581(3):453-6. Corpas et al (2008). Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants. Methods Enzymol. 437:561-74

synthetic Nitroso-L.Glutathione conjugated to protein carrier (PC)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

immunocytochemistry (ICC), ELISA (ELISA) 

to be added when available

data on antibody specificity nad immunolocalization protocol can be found here.

application information

1: 1000 - 1: 5000 (ICH), 1: 5000 - 1: 10 000 (ELISA)

n.a.

GSNO nitroso-L.glutathione

GSNO nitroso-L.glutathione

no confirmed exceptions from predicted reactivity known in the moment

antibody has been used on plant tissue as well as on isolated arteries from a rat

Chaki et al. (29009). Involvement of reactive nitrogen and oxygen species (RNS and ROS) in sunflower-mildew interaction. Plant Cell Physiol. 50:265-279. Corpas et al. (2008). Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants. Methods Enzymol. 437: 561-574.