Plant/Algal cell biology / Conifers

product information

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi.                                                  

KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunofluorescence (IF)

AS07 213 | anti-V-ATPase rabbit antibody

cellular [compartment marker] for plasma membrane

application information

1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF)

95 kDa (Arabidopsis thaliana)

dicots including: Arabidopsis thaliana, Glycine max (weak), Lycopersicon esculentum, Nicotiana tabaccum, Ricinus communis, Spinacia oleracea, monocots inlcuding: Hordeum vulgare, Zea mays, trees: Populus tremula, Pteris vittata (fern), algae: Chlamydmonas reinhardtii,

dicots (including Nicotiana tabacum, Solanum tuberosum, Mesembruanthemum crystallinum); monocots (including Avena sativa, Hordeum vulgare, Oryza sativa, Zea mays), conifers (Pinus thunbergii), mosses (Physocomitrella patens), algae (Dunaliella spp., Ostreococcus spp.), Saccharomyces cerevisiae

no confirmed exceptions from predicted reactivity known in the moment

for additional Western blot detection image please refer to the article below

Page et al. (2009) Two Chlamydomonas CTR Copper Transporters with a Novel Cys-Met Motif Are Localized to the Plasma Membrane and Function in Copper Assimilation. Plant Cell on line

application example

5 µg of total protein from (1) Zea mays lwhole cell, extracted with Protein Extration Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Spinacia oleracea total cell extracted with PEB, (4) Arabidopsis thaliana  were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).