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product information
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| background |
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Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development. |
| immunogen |
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BSA-conjugated synthetic indole 3 acetic acid |
| antibody format |
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rabbit |
polyclonal |
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total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35 |
lyophilized |
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| quantity |
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100 µl, |
for reconstitution add 50 µl, of sterile water and 50 µl of glycerol |
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| storage |
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store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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ELISA (ELISA), immunolocalization (IL) |
| related products |
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AS09 445 | anti-indole- 3- acetic acid (C1') for immunolocalization AS09 420 | anti-indole 3 acetic acid (C1') (for ELISA) AS09 421 | anti-indole 3 acetic acid (N1) collection of antibodies to plant hormones |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5000 - 1: 10 000 (ELISA), 1: 100 (IL) |
| expected | apparent MW |
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n.a. |
| confirmed reactivity |
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Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago sativa (nodules)
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| predicted reactivity |
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dicots and monocots |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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for detailed immunolocalization protocol check here. quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009). |
| selected references |
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Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.
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application example 
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1: 3000 (shown in red color) were used. Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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