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product information
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| background |
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The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. |
| immunogen |
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KLH-conjugated synthetic peptide derived from N-terminal of all known sequences of PsbA protein including Arabidopsis pumila PsbA A4QJR4 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS01 016 | anti-PsbA C-terminal hen antibody AS05 084 | anti-PsbA C-terminal rabbit antibody AS01 016S | PsbA protein standard for quantitation and positive control |
| additional information |
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Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species. |
application information
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| recommended dilution |
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1:1000 with standard ECL, or 1: 10 000 with ECL Advance (WB) |
| expected | apparent MW |
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38 | 28-30 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Synechococcus sp. 7942 |
| predicted reactivity |
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dicots including Nicotiana tabacum, Vitis vinifera, and monocots including Oryza sativa,Triticum aestivum, conifers, brown and red algae, cyanobacteria and diatoms |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel. |
| selected references |
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not available at the moment, antibody released in September 2011 |
application example
5 µg of total protein extracted with Protein Extration Buffer, PEB (AS08 300) from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Chlamydomonas reinhardtii total cell, (4) Synechococcus sp. 7942 total cell, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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