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product information
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| background |
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UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides.
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| immunogen |
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recombinant UGPase overexpressed and purified from E.coli Q43772 |
| antibody format |
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rabbit |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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200 µl, |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1 collection of antibodies to carbohydrate metabolism recommended secondary antibody |
| additional information |
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cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002) |
application information
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| recommended dilution |
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1: 1000 - 1: 3000 with standard ECL (WB) |
| expected | apparent MW |
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51.6 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, C. annuum, C. sativus, F. arundinacea, Hordeum vulgare, L. esculentum, L. chilense, N. tabacum, Oryza sativa, Populus sp., S. tuberosum, S. sogarandinum, |
| predicted reactivity |
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dicots including: Gossipium hirsutum, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots including: Saccharum officinarum, Zea mays, trees: Populus tremula, conifers: Pinus taeda |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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this antibody detectes 1 ng of UGPase in a western blot |
| selected references |
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Yu et al. (2011). Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement and starch accumulation in developing endosperm of Oryza sativa. J of Proteome Res. Nov, ahead of print Estavillo et al. (2011). Evidence for a SAL1-PAP Chloroplast Retrograde Pathway That Functions in Drought and High Light Signaling in Arabidopsis. Plant Cell Nov. 29 (ahead of print). Sullivan et al. (2007). In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1. Mol. Plant-published on-line. Kuras et al. (2007). A specific c-type cytochrome maturation system is required for oxygenic photosynthesi. PNAS 104:9906-9910. |
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A 1-year-old greehouse grown plant was dissectedinto different tissues, which were then used for enzyme assays and immunoblot analyses. Equal amounts of total protein (7.5 μg) were loaded on each lane. SDS-PAGE was run on a 7.5% gel. Immunoblot was done using Amersham PVDF transfer membrane. Primary antibodies against barley UGPase were used in 1: 1000 dilution. Secondary antibodies (Amersham ECL Rabbit IgG, HRP-Linked Whole Antibody from donkey) were used at 1:10 000. ff - female flower, mf - male flower, yl - young leaf, ml - mature leaf, sbk - stem bark, sph - stem phloem and cambium, sxy - stem xylem, rxy - root xylem 
15 µg of total soluble protein extract from leaves and stems of Solanum tuberosum (1), Solanum sogarandinum (2), Lycopersicum esculentum (3), Lycopersicum chilense (4) , Arabidopsis thaliana (5) , Cucumis sativus (6) , Festuca arundinacea (7) , Nicotiana tabacum (8) and Capsicum annuum (9) were separated on 10% SDS-PAGE and blotted onto nitrocellulose . After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1500 in TBST for 1h at room temperature. Following incubation and wash steps, blots were incubated with SIGMA secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugate for 1 hour at a dilution of 1:40000 . Blots were developed with the alkaline phosphatase detection system using NBT/BCIP (SIGMA). Courtesy of Bartosz Szabala, Institute of Plant Genetics, Polish Academy of Science .
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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