Tubulin alpha chain

320 €

AS10 680 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Horderum vulgare, Zea mays

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product information

background

Tubulin alpha (TUA) together with beta tubulin is making up microtubules.

immunogen

KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

200 µl

for reconstitution add 200 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunofluorescence (IF)

related products

AS10 681 | tubulin beta chain

additional information

Please, note that tubulin alpha is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to unable detection on a western blot, analyzed material must contain enough cells with dividing activity.

application information
recommended dilution		1: 1000 with standard ECL (WB), 1: 500 (IF)
expected \| apparent MW		49 \| 52 kDa (Arabidopsis thaliana)
confirmed reactivity		Arabidopsis thaliana, Hordeum vulgare, Zea mays
predicted reactivity		dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Triticum aestivum, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlorella vulgaris, Euglena gracilis, Micromonoas pusilla, Ostreococcus lucimarinus
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		Peptide used to elict this antibody is not present in tubulin beta.
selected references		Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.

application example

western blot

5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3) extracted with Agrisera PEB extraction buffer were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602 ) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Health care). Exposure time was 120 seconds.

immunolocalization

immunolocalization of plant tubulin alpha chain

Tubulin alpha localization in roots of Arabidopsis thaliana. Tubulin alpha (red), nucleus (DAPI white). Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin alpha 1: 500. Secondary antibody: goat anti-rabbit IgG Alexa conjugated (red color), dilution 1: 500. Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

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