AtFH14 | Formin-like protein 14 (100 ug)

378 €

AS11 1752 | clonality: monoclonal | host: mouse | reactivity:Arabidopsis thaliana

product information

background

Formins are known to regulate microfilaments and associate with microtubles. Formin 14 acts as a linking protein between microtubules and microfilaments and plays an important role in the process of plant cell division. Synonymes:

immunogen

recombinant formin of Arabidopsis thaliana, amino acids 340-520, NP_17446

antibody format

mouse

monoclonal

IgG1

liquid

quantity

100 µg

storage

store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Antibodyis stable one year from the date of shipment.

tested applications

western blot (WB), immunofluorescence (IF)

related products

AS11 1750 | Anti-AtFH14 | Formin-like protein 14 (50 ug)

AS10 702 | Anti-actin-11 mouse antibody

AS10 681 | Anti-tubulin beta chain rabbit antibody

AS10 680 | Anti-tubulin alpha chain rabbit antibody

additional information

to be added when available

application information
recommended dilution		1 : 1000 - 1: 2000 with standard ECL (WB), 1:100 - 1: 500 (IF)
expected \| apparent MW		115 kDa
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		Arabidopsis thaliana
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		Li et al. (2010). The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division. Plant Cell (8):2710-2726.

application example

immunofluorescence detection using anti-formin antibodies

Arabidopsis thaliana suspension cells were fixed in 4% (w/v) paraformaldehyde in PEM buffer (50 mM PIPES, 5 mM EGTA, 5 mM MgSO4, 0.1 M mannitol, pH 6.9) for 30 min at room temperature. Wash has been done with with PEM buffer and treated with 0.5% pectolyase and 1% cellulase R-10 in PEM buffer for 10 min at RT. Blocking was done in (w/v) BSA in PEM buffer. Incubation with primary antibodies at a dilution of 1: 100 has been done overnight at 48°C in a dark and moist chamber.

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