Patatin

336 €

AS12 1842 | clonality: polyclonal | host: rabbit | reactivity: Solanum tuberosum

PRODUCT INFORMATION IN PDF

product information

background

Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges.

immunogen

KLH-conjugated synthetic peptide derived from a C-terminal part of 36 known isoforms of patatin from Solanum tuberosum including: Q3YJS9, Q3YJT0, Q42502, Q3YJT2

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

200 µl

for reconstitution add 200 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

collection of antibodies to Solanum tuberosum

additional information

to be added when available

application information
recommended dilution		1 : 2000 with standard ECL (WB)
expected \| apparent MW		40-42 kDa
confirmed reactivity		Solanum tuberosum
predicted reactivity		Solanum tuberosum
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in October 2012.

application example

western blot detection using anti-patatin antibody

75 µg of total protein from Solanum tuberosum v. Nicola extracted with 20 mM TRIS PH-8.5, 10 mM thiourea,10mM CaCl2, 5mM DTT, 1 mM PMSF, 1%PVPP were separated on 4-20 % SDS-PAGE and blotted 1.5h to PVDF. Blots were blocked with 5% milk powder in T-TBS (2.5gr of powder in 50 ml T-TBS) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed 6 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in blocking solution for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy of Dr. Paula Teper-Bamnolker and Dr. Dani Eshel, The Volcani Center, Israel

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