RBR1 | retinoblastoma related protein

PRODUCT INFORMATION IN PDF

product information background   The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR immunogen   recombinant C-terminal fragment consisting of 236 amino acids  of Arabidopsis thaliana retinoblastoma protein Q9LKZ3, locus At3g12280 antibody format   hen polyclonal total IgY in PBS, pH 8.5 + 0.02 % sodium azide liquid quantity   50 µl storage   store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications   western blot (WB), immunoprecipiation (IP) related products   collection of antibodies for plant developmental biology additional information   total IgY concentration is 30.8 mg/ml

application information
recommended dilution		1 : 6000 with standard ECL (WB),  (IP)
expected | apparent MW		112 kDa
confirmed reactivity		Arabidopsis thaliana, Medicago sativa
predicted reactivity		dicots: Camelia sinensis, Chenopodium rubrum, Pisum sativum, Scutelaria baicalensis, monocots: Cocos nucifera, Hordeum vulgare, Zea mays, Oryza sativa, trees: Populus tremula
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		antibody is not suitable for immunolocalization.
selected references		Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7. Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.

application example



35 µg of total proteins from Arabidopsis thaliana Col-0 cell suspension culture (1) or one week old seedlings (2) as well as 3.5ng of purified GST-RBR1 fusion protein (3) were separated on 8% Laemmli SDS polyacrylamide gels and blotted onto PVDF membrane overnight. Filters were blocked in 5% milk powder in TBS-0.05% Tween 20 (TBS-T) for 2 hours then probed with anti-RBR1 antibody (1:6000,  2 hours at RT) and HRP-conjugated rabbit anti-chicken IgY (PIERCE) secondary antibody (1: 20000, 1 hour at RT) in TBS-T containing 5% milk powder. After each antibody incubation steps filters were washed with TBS-T, TBS-T containing 2% milk powder, TBS-T for 10 min each on a rocking platform. Signal was developed with Super Signal West Pico chemiluminescent substrate (PIERCE) and visualized by exposing to a film (Agfa Cronex 5) for 5 min. 
Courtesy Dr. Laszlo Bako, Umeå Plant Science Center, Sweden

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