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RBR1 | retinoblastoma related protein

320 €

AS11 1627   | clonality: polyclonal  |  host: rabbit  |  reactivity:Arabiodpis thaliana, Medicago sativa

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Item No:
AS11 1627

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product information
background  

The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR

immunogen  

recombinant C-terminal fragment consisting of 236 amino acids  of Arabidopsis thaliana retinoblastoma protein Q9LKZ3, locus At3g12280

antibody format  

hen

polyclonal

total IgY in PBS, pH 8.5 + 0.02 % sodium azide

liquid

quantity  

50 µl

storage  

store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), immunoprecipiation (IP)

related products   collection of antibodies for plant developmental biology
additional information  

total IgY concentration is 30.8 mg/ml

application information
recommended dilution  

1 : 6000 with standard ECL (WB),  (IP)

expected | apparent MW  

112 kDa

confirmed reactivity  

Arabidopsis thaliana, Medicago sativa

predicted reactivity   dicots: Camelia sinensis, Chenopodium rubrum, Pisum sativum, Scutelaria baicalensis, monocots: Cocos nucifera, Hordeum vulgare, Zea mays, Oryza sativa, trees: Populus tremula
not reactive in  

no confirmed exceptions from predicted reactivity are currently known

additional information  

antibody is not suitable for immunolocalization.

selected references   Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7.

Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.

 


application example

western blot detection of plant retinoblastoma protein

35 µg of total proteins from Arabidopsis thaliana Col-0 cell suspension culture (1) or one week old seedlings (2) as well as 3.5ng of purified GST-RBR1 fusion protein (3) were separated on 8% Laemmli SDS polyacrylamide gels and blotted onto PVDF membrane overnight. Filters were blocked in 5% milk powder in TBS-0.05% Tween 20 (TBS-T) for 2 hours then probed with anti-RBR1 antibody (1:6000,  2 hours at RT) and HRP-conjugated rabbit anti-chicken IgY (PIERCE) secondary antibody (1: 20000, 1 hour at RT) in TBS-T containing 5% milk powder. After each antibody incubation steps filters were washed with TBS-T, TBS-T containing 2% milk powder, TBS-T for 10 min each on a rocking platform. Signal was developed with Super Signal West Pico chemiluminescent substrate (PIERCE) and visualized by exposing to a film (Agfa Cronex 5) for 5 min. 
Courtesy Dr. Laszlo Bako, Umeå Plant Science Center, Sweden

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com