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product information
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| background |
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The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR |
| immunogen |
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recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein Q9LKZ3, locus At3g12280 |
| antibody format |
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hen |
polyclonal |
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total IgY in PBS, pH 8.5 + 0.02 % sodium azide |
liquid |
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| quantity |
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| storage |
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store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunoprecipiation (IP) |
| related products |
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collection of antibodies for plant developmental biology |
| additional information |
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total IgY concentration is 30.8 mg/ml |
application information
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| recommended dilution |
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1 : 6000 with standard ECL (WB), (IP) |
| expected | apparent MW |
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112 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Medicago sativa
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| predicted reactivity |
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dicots: Camelia sinensis, Chenopodium rubrum, Pisum sativum, Scutelaria baicalensis, monocots: Cocos nucifera, Hordeum vulgare, Zea mays, Oryza sativa, trees: Populus tremula |
| not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
| additional information |
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antibody is not suitable for immunolocalization. |
| selected references |
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Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168. Horváth et al. (2006). EBP1 regulates organ size through cell growth and proliferation in plants. EMBO J 25(20): 4909-4920. |
application example
35 µg of total proteins from Arabidopsis thaliana Col-0 cell suspension culture (1) or one week old seedlings (2) as well as 3.5ng of purified GST-RBR1 fusion protein (3) were separated on 8% Laemmli SDS polyacrylamide gels and blotted onto PVDF membrane overnight. Filters were blocked in 5% milk powder in TBS-0.05% Tween 20 (TBS-T) for 2 hours then probed with anti-RBR1 antibody (1:6000, 2 hours at RT) and HRP-conjugated rabbit anti-chicken IgY (PIERCE) secondary antibody (1: 20000, 1 hour at RT) in TBS-T containing 5% milk powder. After each antibody incubation steps filters were washed with TBS-T, TBS-T containing 2% milk powder, TBS-T for 10 min each on a rocking platform. Signal was developed with Super Signal West Pico chemiluminescent substrate (PIERCE) and visualized by exposing to a film (Agfa Cronex 5) for 5 min.
Courtesy Dr. Laszlo Bako, Umeå Plant Science Center, Sweden
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