|
product information
|
| background |
|
Sporamin is a storage protein of tuberous roots of sweet potato and accounts for more than 80 % of the soluble protein content. Protein is localized to vacuole. |
| immunogen |
|
native protein purified from sweet potato taproot |
| antibody format |
|
rabbit |
polyclonal |
|
total IgG |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl, of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
antibodies to vacuolar proteins |
| additional information |
|
contains less than 0.1 % sodium azide as preservative |
application information
|
| recommended dilution |
|
1 : 2000 with standard ECL (WB) |
| expected | apparent MW |
|
25 kDa (when fresh DTT is used in sample buffer) |
| confirmed reactivity |
|
Ipomoea batatas
|
| predicted reactivity |
|
Ipomoea sp. |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
This antibody is recognizing both, sporamin A and B. |
| selected references |
|
Maeshima et al. (1985) Characterization of major proteins in sweet potato tuberous roots. Phytochemistry, 24: 1899-1902.
Hattori et al. (1985) Molecular cloning and nucleotide sequence of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Molecular Biology, 5: 313-320. |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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