Plant/Algal cell antibodies / Developmental biology

product information

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), ELISA (ELISA)

n.a.

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

application information

1: 1000 (WB), 1: 10 000 (ELISA)

23.7

Triticum aestivum

Triticum monococcum

no confirmed exceptions from predicted reactivity known in the moment

there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

to be added when available