Plant/Algal cell antibodies / Developmental biology

product information

Xyloglucan endotransglucosylases (Xet) are a class of enzymes capable of catalyzing the molecular grafting between xyloglucans and/or the endotype hydrolysis of a xyloglucan molecule in plant cell wall. XET protein resides in the primary cell wall and at least some of the enzyme molecules are covalently attached to the xyloglucan and dissociate from it when another xyloglucan molecule is available to make a new glycosidic bond. The enzyme can be easily eluted from cell wall with low salt buffers.

recombinant Xet protein derived from Pupulus tremula x tremuloides sequence A2TEJ3

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunohistochemistry (IHC)

AS05 091 | anti-Xet hen antibody

AS08 319 | anti-Xet rabbit antibody with reactivity to barley

No signal was so far obtained with this antibody using a transmission electron microscopy.

application information

1: 1000 with standard ECL (WB), 1:10 (IHC)

31.9 kDa

Populus tremula x Populus tremuloides

dicots including Arabidopsis thaliana, Asparagus officinalis, Cucumis melo, Cucumis sativum, Malus sylvestris, Solanum lycopersicum

no confirmed exceptions from predicted reactivity known at the moment

to be added when available

Bourquin et al. (2002).  Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues. Plant Cell 14: 3073-3088.