Plant/Algal cell antibodies / Developmental biology

product information

Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-β-d-glucans, are fundamental to cell wall properties.  These interactions have been assumed to be non-covalent in nature in most cases.  A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-β-d-glucans.  It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) ELISA (ELISA)

AS05 080 | anti-Xet rabbit antibody

to be added when available

application information

1: 500 with standard ECL (WB) , 1: 5000 (ELISA)

31.5 | 33 kDa

Hordeum vulgare

Triticum aestivum, Oryza sativa

Zea mays

to be added when available

Hrmova et al. (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)-β-d-glucans. J. Biol. Chem. 82: 12951-12962.