8-Hydroxyguanosine | DNA/RNA oxidative damage (100 µg)

345 €

AS10 708-100 | clonality: monoclonal | host: mouse | reactivity: hydroxy guanosine

PRODUCT INFORMATION IN PDF

product information

background

Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

immunogen

8-hydroxy-guanosine-BSA and – casein conjugates

antibody format

mouse

monoclonal

IgG2A (clone 15A3)

liquid

quantity

100 µg

storage

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

ELISA (ELISA), immunostaining on frozen tissue and paraffin-embedded ((IHC-Fr-P), affinity purification (AP)

related products

AS10 708-25 | hydroxy guanosine monoclonal antibody, 25 µg

additional information

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml

application information
recommended dilution		needs to be determined for each application
expected \| apparent MW		n.a.
confirmed reactivity		Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
predicted reactivity		does not apply
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		protocol for immunostaining using this antibody can be found here
selected references		Pilger & Rudiger (2006). 8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. Int Arch Occup Environ Health. 1: 1-15. Kowluru et al. (2006). Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy.Invest Ophthalmol Vis Sci 4: 1594-9. Kim et al. (2003). Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells. Carcinogenesis 2: 235-241. Cui et al. (2000). Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain. Faseb J. 14(7): 955-67.

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