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product information
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| background |
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Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). |
| immunogen |
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acetylated KLH |
| antibody format |
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mouse |
monoclonal |
IgG1 (clone 7F8) |
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liquid |
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| quantity |
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| storage |
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store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), binds Protein G |
| related products |
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AS10 707-25 | acetylated lysine monoclonal antibody, 25 µg |
| additional information |
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protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues. |
application information
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| recommended dilution |
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needs to be determined for each application |
| expected | apparent MW |
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n.a. |
| confirmed reactivity |
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bovine, avian |
| predicted reactivity |
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higher plants |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system |
| selected references |
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Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218. |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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