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product information
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| background |
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AtWRKY6 is a transcription factor involved in the control of processes related to senescence and pathogen defense. This protein is interacting with the W box (5'-(T)TGAC[CT]-3'). Alternative names: WRKY transcription factor 6, WRKY DNA-binding protein 6.
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| immunogen |
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recombinant part of Arabidopsis thaliana WRKY transcription factor 6 Q9C519, locus At1g62300 |
| antibody format |
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rabbit |
polyclonal |
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affinity purified serum |
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| quantity |
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| storage |
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store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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antibodies to DNA/RNA/cell cycle |
| additional information |
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antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth |
application information
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| recommended dilution |
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1 : 1000 with standard ECL (WB) |
| expected | apparent MW |
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60.5 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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Brassica napus, Coffea sp. , Pisum sativum
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| not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in March 2012 |
application example
50 µg of total protein from Arabidopsis thaliana wilde type roots (WT) and wrky6 knock out mutant were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 1% BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 80 seconds. Courtesy Dr. Tzyy-Jen Chiou, Academia Sinica, Taiwan
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