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product information
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| background |
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Programmed cell death (PCD) is a fundamentally important biological process required to maintain the integrity and homeostasis of multicellular organisms during normal development and as a response to adverse environments. Cdc2 kinase, plays a major role in driving the cell cycle. Synonymes (for Arabidopsis thaliana): CDC2A, CDKA-1 |
| immunogen |
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KLH-conjugated synthetic peptide derived form Zea mays CDC2 sequence P23111, well conserved in other plant species |
| antibody format |
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rabbit |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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100 µl, |
for reconstitution add 100 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunolocalization (IL) |
| related products |
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collection of antibodies for DNA/RNA metabolism and cell cycle |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 2000 - 1 : 10 000 with standard ECL (WB), 1: 500 (IL) |
| expected | apparent MW |
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34-36 (Zea mays) |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Zea mays, Vicia faba
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| predicted reactivity |
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dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Triticum aestivum, trees:Pinus contorta, Populus balsamifera, moss: Physcomitrella patens |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
application example
Seeds of field bean (Vicia faba L. subsp. minor var. Nadwiślański; DANKO Group; Sobiejuchy) were sterilized
using sodium hypochlorite (0.3% v/v) and germinated in Petri dishes on wetted filter paper at room
temperature. At 4 d after imbibition, dark-grown seedlings with primary roots 25±5 mm long were selected for experiments. During incubations roots were oriented horizontally in a humid chamber and aerated continuously on a rotary water-bath shaker (30 rpm) at 23°C.
Immunocytochemical assays were performed according to the method prescribed earlier (Rybaczek and
Maszewski 2006). Excised apical parts of roots (1.5 mm long) were fixed for 45 min (18°C) in PBS-buffered
3.7% paraformaldehyde, washed several times with PBS and placed in a citric acid-buffered digestion solution
(pH 5.0; 37°C for 45 min) containing 2.5% pectinase (Fluka), 2.5% cellulase (Onozuka R-10; Serva) and 2.5% pectoliase (ICN). After removing the digestion solution, root tips were washed 3 times in PBS, rinsed with distilled water and squashed onto Super Frost Plus glass slides (Menzel-Gläser). Air-dried slides were
pretreated with PBS-buffered 5% BSA at 20°C for 50 min and incubated overnight in a humidified atmosphere
(4°C) with rabbit antibody raised against CDC2 (Agrisera), dissolved in PBS containing 1% BSA (at a dilution
of 1:500). Following incubation, slides were washed 3 times with PBS and incubated for 1 h (18°C) with
secondary goat anti-rabbit IgG DyLight®488 antibody (AS09 633, Agrisera; 1:3000). Nuclear DNA was stained with 4’,6-diamidino-2-phenyl-indole (DAPI, 0.4 μg/ml; Sigma-Aldrich). Following washing with PBS, slides were air dried and embedded in Vectashield Mounting Media for Fluorescence (Vector Laboratories). Observations were made using Optiphot-2 fluorescence microscope (Nikon) equipped with B-2A filter (blue light; λ ≈ 495 nm) for DyLight-conjugated antibodies and UV-2A filter (UV light; λ ≈ 365 nm) for DAPI. All images were recorded at exactly the same time of integration using DXM 1200 CCD camera. Courtesy Dr. Dorota Rybaczek, Lodz University, Poland
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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