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product information
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| background |
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EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A. |
| immunogen |
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full length, recombinant EF1A of Arabidopsis thaliana P13905 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2 AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2 AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2 AS07 265 | eEF1b | elongation factor eEF1b-beta protein AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2 |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 1000 with standard ECL (WB) |
| expected | apparent MW |
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49.5 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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dicots including: Brassica napus, Solanum tuberosum, Sorghum bicolor,monocots including: Oryza sativa, Zea mays, trees: Populus trichocarpa, Picea sitchensis |
| not reactive in |
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recombinant wheat EF1A |
| additional information |
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to be added when available |
| selected references |
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to be added when available. Antibody released in February 2011. |
application example
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22°C (3) or heat treated at 38°C for 2 hours prior to protein extraction (4). As positive control 10 ng of recombinant AtEF1A (2) were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1000 for O/N 4°C with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody, anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602, diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturer's instructions. Exposure time was 5 seconds.
Lane (1) with no signal: recombinant Triticum spp. EF1A. Courtesy Dr. Eman Basha, Arizona State University
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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