HDT1 | histone deacetylase

345 €

AS11 1792 | clonality: polyclonal | host: rabbit | predicted reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

product information

background

HDT1 (HD2A) is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in cell cycle progression, transcriptional regulation and developmental events. This protein is also involved in rRNA gene silencing in nucleolar dominance. Synonymes:HD-tuins protein 1, Histone deacetylase 2a.

immunogen

recombinant part of Arabidopsis thaliana HDT1 Q9FVE6, At3g44750

antibody format

rabbit

polyclonal

affinity purified serum

quantity

100 µg

storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS11 1753 | histone deacetylase| HDT3

collection of antibodies for developmental biology

additional information

antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth

application information
recommended dilution		1 : 1000 with standard ECL (WB)
expected \| apparent MW		26.4 kDa
confirmed reactivity		to be determined
predicted reactivity		Arabidopsis thaliana
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available

application example

50 µg of Arabidopsis T87 nuclear proteins, extracted with Nuclei Lysis Buffer (1% SDS, 50mM Tris-HCl, 10mM EDTA, Complete EDTA-free) or 50 µg of total Arabidopsis T87 proteins, extracted with Laemmli Buffer from grinded in liquid nitrogen material, were separated on 12 % SDS-PAGE and blotted 1,5h to PVDF membrane (WestranS 0,20 µm, Whatman). Blots were blocked with 5% fat free milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 2,5% fat-free milk in TBST overnight at 40°C with agitation. The antibody solution was decanted and the blot was washed with TBST three times for 10 min. and blocked in 10% fat-free milk in TBST for 10 min. Next, blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Sigma) diluted to 1:5 000 in 5% fat-free milk in TBST for 2h at RT with agitation. The blot was washed six times for 10 min. with TBST and developed for 5 min with ECL+ (Amersham) according to the manufacturers instructions. Exposure time was 1 min.

Courtesy of Msc. Daniel Buszewicz, PAN, Poland

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