Spermine

Spermine is a positively charged polyamine found in a wide variety of tissues. Alternative names: gerontine, musculamine, neuridine

Synthetic spermine conjugated to BSA

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

indirect ELISA (ELISA) with conjugated spermine

AS09 598 | anti-ornithine rabbit antibodies

Specificity has been determined using ELISA competition experiments using conjugate of spermine

Compounds                                 Cross-reactivity ratio (a)

Spermine-GA-BSA                                     1

Spermidine-GA-BSA                                1/3
Ornithine-GA-BSA                                   1/500
Diamino-Pentane-G-BSA                         1/700

(a) : Spermine-GA-BSA concentration/other conjugated polyamine concentration at half displacement
G = Glutaraldehyde, GA = Glutaric Anhydride, BSA = Bovine Serum Albumin.

Antibodies have very weak reactivity to spermidine and no reactivity to putrescine.