Plant/Algal cell antibodies / Environmental stress / Cold stress

product information

Cor14b is a nuclear encoded protein localized in chloroplast whose expression level has been associated with frost and photoinhibition tolerance in monocots.

KLH-conjugated synthetic peptide conserved in cor14b sequence of Hordeum vulgare, cor14c Triticum aestivum, REP13 of Secale cereale 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

collection of antibodies to various plant stress proteins

to be added when available

application information

1: 10 000 with standard ECL (WB)

16 kDa

Hordeum vulgare

monocts

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Rapacz et al. (2008). The effect of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high light treatment in cold. Ann. Bot. 101: 689-699.

application example

Total cell extract of (1-3) Hordeum vulgare  after 1 day of cold acclimation (4-6) Hordeum vulgare  after 6 hours of cold acclimation, (7-9) Hordeum vulgare  non-acclimated plants were separated on  SDS-PAGE. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation.  Blots were developed using ECL.