Plant/Algal cell antibodies / Environmental stress / Pathogen attack

product information

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

purified tobacco class I, basic  ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunolocalization (IL)

AS07 207 | anti-PR-3 | CHN | class I chitinase

collection of antibodies to other proteins involved in stress response

for more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51

This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51. 

application information

8 ug/ml with standard ECL (WB)

37 | 33 kDa

Nicotiana tabacum, Populus sp. 

dicots including: Solanum lycopersicum, Solanum tuberosum,

Arabidopsis thaliana

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.

This antibody is purified by affinity chromarography on Portein G.

Sticher,L., Hinz,U., Meyer,A.D., and Meins,F.Jr. (1992). Intracellular transport and processing of a tobacco vacuolar β -1,3-glucanase. Planta 188, 559-565.

Beffa et al. (1993). Physiological compensation in antisense transformants: Specific induction of an ersatz glucan endo-1,3- β -glucosidase in plants infected with necrotizing viruses. Proc. Natl. Acad. Sci. U. S. A 90, 8792-8796.