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product information
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| background |
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Hellethionins belong to a family of plant thionins, a small-sized multiple-cysteine peptides that are involved in the plant defence against pathogens. The antimicrobial activity and cytotoxicity of thionins have led to the in agriculture transgenic plants containing thionin genes can enhance pathogen resistance. |
| immunogen |
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ovalbumin conjugated mixture of hellethionins A, B, C, D, E and G extracted from Helleborus purpurascens
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| antibody format |
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| rabbit |
polyclonal |
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serum |
liquid |
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| quantity |
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| storage |
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Short term 4°C. Long term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide. |
| tested applications |
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ELISA (ELISA), western blot (WB) |
| related products |
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AS10 1622 | anti-hellethionin, larger pack size
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| additional information |
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Antibody reacts with hellethionins A, B, C, D, E and G from H. purpurascens. |
application information
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| recommended dilution |
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1 : 256 000 (ELISA), 1: 500 with standard ECL (WB) |
| expected | apparent MW |
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does not apply |
| confirmed reactivity |
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H. purpurascens |
| predicted reactivity |
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n.a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Milbradt et al. (2003). Structural characterisation of hellethionins from Helleborus purpurascens. Biochemistry 42, 2404 – 24112. Silverstein et al. (2007). Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants. Plant J. 51, 262 – 280. |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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