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product information
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| background |
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Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Synonymes: linoleate, oxygen oxidoreductase. |
| immunogen |
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native lipoxygenase, type I-B, purified from Glycine max (Sigma, product number L7395) P08170 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), immunohistochemistry (IHC) |
| related products |
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AS07 258 | anti-LOX-C | chloroplastic lipoxygenase AS08 340 | anti-LOX2 | lipoxygenase 2 protein |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1:1000 for alkaline phosphatase (WB), 1: 1000 (IHC) |
| expected | apparent MW |
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54 (subunit), 108 (native enzyme) |
| confirmed reactivity |
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Arabidopsis thaliana, Glycine max, Lathyrus undulatus, Malus x domestica
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| predicted reactivity |
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n.a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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IHC: secondary antibodies goat anti-rabbit conjugated with 10 nm gold particle (GAR-gold, Sigma) has been used. Secondary antibody dilution in PBS-BSA was 1: 50 |
| selected references |
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Vardar and Ünal (2011). Immunolocalization of Lipoxygenase in the Anther Wall Cells of Lathyrus undulatus Boiss. during Programmed Cell Death. Not Bot Hort Agrobot Cluj 39(1):71-78.
Skórzyńska-Polit et al. (2006). The activity and localization of lipoxygenases in Arabidopsis thaliana under cadmium and copper stresses. Plant Growth Regul 48:29–39 |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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