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PIP2;3 | aquaporin, plasma membrane intrinistic protein 2-3

440 €

AS09 506 | clonality: polyclonal | host: rabbit | reactivity: Oryza sativa, Zea mays

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AS09 506

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product information
background

PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3

immunogen

KLH-conjugated peptide derived from Oryza sativa PIP2;3  Q7XUA6

host

rabbit

clonality

polyclonal,

clone
purity

serum,

format
quantity 100 ĩl
reconstitution
storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications

ELISA (ELISA), western blot (WB), immunogold (IG)

related products

collection of antibodies to plasma membrane proteins

AS09 505 | anti-PIP1s | aquaporin

AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5

additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

Protocol for isolation of plasma membrane can be found here.

application information
recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IG)

expected | apparent MW

31.9 | 26-29 kDa

confirmed reactivity

Oryza sativa, Zea mays

predicted reactivity

monocots 

not reactive in

Arabidopsis thaliana

additional information

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

selected references

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39. 


application example

 

 

0.5 µg of crude membrane fraction/lane from Oryza sativa  L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;1 antibodies (AS09 506, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

 

  western blot detection using anti-PIP2;1

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