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PIP2;3 | aquaporin, plasma membrane intrinistic protein 2-3

416 €

AS09 506 | clonality: polyclonal | host: rabbit | reactivity: Oryza sativa, Zea mays

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AS09 506

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product information
background  

PIP2;1 fuctions as a silicon transporter which helps plants to overcome abiotic and biotic stress. Alternative names: aquaporin NIP2-1,NOD26-like intrinsic protein 2-1 OsNIP2;1, Silicon transporter LSI1, Low silicon protein 1

immunogen  

KLH-conjugated peptide derived from Oryza sativa PIP2;3  Q7XUA6

antibody format  

rabbit

polyclonal,

serum,

quantity  

100 µl

storage  

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications  

ELISA (ELISA), western blot (WB), immunogold (IG)

related products  

collection of antibodies to plasma membrane proteins

AS09 505 | anti-PIP1s | aquaporin

AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5

additional information  

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

Protocol for isolation of plasma membrane can be found here.

application information
recommended dilution  

1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IG)

expected | apparent MW  

31.9 | 26-29 kDa

confirmed reactivity  

Oryza sativa, Zea mays

predicted reactivity  

monocots 

not reactive in  

Arabidopsis thaliana

additional information  

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

selected references  

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39. 


application example

 

 

0.5 µg of crude membrane fraction/lane from Oryza sativa  L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;1 antibodies (AS09 506, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

 

  western blot detection using anti-PIP2;1

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com

Do you have a matching secondary antibody?

AS09 602 | Goat anti-rabbit IgG (H&L), HRP conjugated

AS09 607 | Goat anti-rabbit IgG (H&L), ALP conjugated 

AS10 1489 | Rabbit anti-Chicken IgY (H&L), HRP conjugated 

AS09 606 | Goat anti-chicken IgY (H&L), ALP conjugated

For a complete list of Agrisera secondary antibodies


Detection reagents:

AS14 ECL-100 | AgriseraECL Bright 
AgriseraECL Bright for Western Blot detection is a high quality substrate for detection of horseradish peroxidase enzyme activity at a femtogram level. Its a ready to use 2 component system with low background and superior signal to nouse ratios.

AS14 TMB-HRP | AgriseraTMB HRP Peroxidase Microwell Substrate (100 ml) 
TMB based, one component, especially formulated with extreme sensitivity, HRP substrate for microwell application.