|
product information
|
| background |
|
Sub1C is a protein which is involved in rice tolerance to submergence. |
| immunogen |
|
KLH-conjugated synthetic peptide derived from Oryza sativa Sub1C. Chosen peptide is convered in all 6 isoforms (C-1 to C-6). |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl, of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS11 1769 | Sub1A |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
|
1 : 5 000 with standard ECL (WB) |
| expected | apparent MW |
|
26 kDa |
| confirmed reactivity |
|
Oryza sativa
|
| predicted reactivity |
|
Oryza sativa |
| not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
| additional information |
|
to be added when available |
| selected references |
|
to be added when available, antibody released in December 2011. |
application example 
Ten days old seedlings of Oryza sativa (cv Nipponbare) were submerged under water for 3 days, epigeal biomass was collected from control and treated samples for protein extraction. 25 µg of total protein were extracted with SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2 % SDS, 1,5 % Sucrose). Proteins were separated on 4-12 % SDS-PAGE (Criterion XT Precast Gel, Biorad) and blotted 7 min to PVDF with Trans-Blot Turbo Transferase System (Biorad). Blot was blocked with TTBS-milk for 1 h at RT with shaking. Blot was incubated in the primary antibody at a dilution of 1:5 000 overnight at 4 °C with shaking. The blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TTBS at RT with shaking. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602 Agrisera) diluted 1:20 000 in TTBS-milk for 45 min at RT with shaking. The blot was washed as above and developed for 5 min with ECL (LiteAblot Turbo, Euroclone) according to the manufacturers instructions. Total exposure time was 3 min. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Arrow marks Sub1C protein. Courtesy of Dr. Viet-The Ho, Scuola Superiore Sant'Anna, Italy
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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