Plant/Algal cell antibodies / Environmental stress / Oxidative stress

product information

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

ascorbate oxidase purified from Cucurbita sp.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Western blot (WB), ELISA (ELISA), immunohistochemistry (IHC)

AS06 184 | anti-ascorbate oxidase rabbit antibody

AS09 383 | anti-ascrobate oxidase biotinylated rabbit antibody

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography. 

application information

1: 1000 (WB), 1: 1000 - 1: 30 000 (ELISA)

61 | 45 kDa  (Cucurbita pepo)

Cucurbita sp.

dicots including Glycine max, monocots including Oryza sativa

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available