Plant/Algal cell antibodies / Environmental stress / Oxidative stress

product information

Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.

KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipitation (IP)

AS06 184 | anti-AO | apoplastic ascorbate oxidase

AS09 384 | anti-AO | ascorbate oxidase

AS09 383 | anti-AO | ascorbate oxidase, biotinylated antibody

AS08 368 | anti-APX | ascorbate peroxidase

total IgG concentration is 3 µg/µl

application information

1:10 000 with standard ECL (WB), 2 µg (IP)

28 kDa

Arabidopsis thaliana (immunohistochemistry only) D. salina, A. toxicaria, Horderum vulgare, Pisum sativum, S. lycopersicum, , Solanum tuberosum, P. silvestris, Zea mays

dicots including: Brassica. juncea, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum,, Solanum tuberosum,  Vitis vinifera, monocots including: Oryza sativa, trees: Pinus pinaster

no confirmed exceptions from predicted reactivity known in the moment

for western blot detection images - please check the publication below

Bai et al. (2011). Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation. PLoSONE.

Mittova et al. (2004). The role of mitochondrial APX isoforms in determining sensitivity to salt stress in two tomato species, Lycopersicon esculentum and L. pennellii. Plant Cell Environ. 27: 237-250.

application example

(A) , (B)- control antibody, anti-PIN1, (C,D) immunolocalization using anti-cAPX antibodies. 

Courtesy of Dr. Taras Pasternak