Plant/Algal cell antibodies / Environmental stress / Oxidative stress

product information

Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for  plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).

maltose binding protein (MBP) fusion of Zea mays GR, O64409

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunolocalization (IL), immunoprecipitation (IP)

AS06 183 | anti-GS, glutathione synthase antibodies

collection of antibodies to stress proteins

total IgG concentration is 7 µg/ µl

application information

1: 5000 with standard ECL (WB), needs to be optimized, 2 µg (IP)

54 kDa

Nicotiana tabacum, Silene vulgaris, Solanum tuberosum, Zea mays

dicots including: Arabidopsis thaliana, Brassica rapa, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, trees: Populus balsamifera

no confirmed exceptions from predicted reactivity known in the moment

This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.

Yoon et al. (2012). Glutathione reductase from Brassica rapa affects tolerance and the redox state but not fermentation ability in response to oxidative stress in genetically modified Saccharomyces cerevisiae. wORL j mICROBIOL. bIOTECHNOL.

Bai et al. (2011). Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation. PLoSONE.

Mittova et al. (2003). Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato. FEBS Lett. 554: 417-421.

application example

10 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Nicotiana tabaccum leaf extracted with PEB, (3) Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5) Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtii total cell extracted with PEB,  (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for  30 seconds  with WEST PICO reagent according the manufacturers instructions.