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Plant/Algal cell antibodies / Environmental stress / Oxidative stress


GSH | glutathione

Art no: AS09 595
Price: 360
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product information

background  

Glutathione (GSH) is a nucleophilic tripeptide which helps to protect cells from reactive oxigen species such as free radicals and peroxides.

immunogen  

Synthetic glutathione  conjugated to BSA protein carrier.

antibody format  

rat

polyclonal

total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35

lyophilized

quantity  

100 µl,

for reconstitution add 50 µl, of sterile water and 50 µl of glycerol

storage  

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

ELISA (ELISA), immunohistochemistry (IHC)

related products  

AS09 594 | anti-GSH | glutathione rabbit antibodies

 

additional information  

Specificity has been determined using ELISA competition experiments using conjugate of glutathione-glutaraldehyde protein.

Compounds                                Cross-reactivity ratio (a)

Glutathione-G-BSA                               1
Cysteine-G-BSA                                    1/>10,000
Glutamate-G-BSA                                 1/>10,000
Glycine-G-BSA                                      1/>10,000

(a) : Glutathione-G-BSA concentration /unconjugated or conjugated analogs concentration at half displacement.

 

G = Glutaraldehyde, BSA = Bovine Serum Albumin

application information

recommended dilution  

1: 2000 - 1: 5000 (ELISA), 1: 500 - 1: 1 000 (IHC)

expected | apparent MW  

n.a.

confirmed reactivity  

glutathione

predicted reactivity  

glutathione

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

for example of immunocytochemistry protocol check here.

selected references  

Amara et al. (1994). Antibodies to reduced glutathione.Brain Res. 659:237-242.



||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com



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