Glutathione (GSH) is a nucleophilic tripeptide which helps to protect cells from reactive oxigen species such as free radicals and peroxides.
immunogen
Synthetic glutathione conjugated to BSA protein carrier.
antibody format
rabbit
polyclonal
total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35
lyophilized
quantity
100 µl,
for reconstitution add 50 µl, of sterile water and 50 µl of glycerol
storage
store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
L.Cysteine-G-BSA: 1/>50 000 L. Glutamate-G-BSA: 1/>50 000
(a) : Glutathione-G-BSA concentration /unconjugated or conjugated analogs concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
application information
recommended dilution
1: 1000 - 1: 5 000 (IHC)
expected | apparent MW
n.a.
confirmed reactivity
glutathione
predicted reactivity
glutathione
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
for example of immunocytochemistry protocol check here.
selected references
Gerdon et al. (2005). Quartz crystal microbalance detection of glutathione-protected nanoclusters using antibody recognition. Anal. Chem. 1:304.310.
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