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Plant/Algal cell antibodies / Environmental stress / Oxidative stress


HRP | horseradish peroxidase

Art no: AS09 548
Price: 185
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product information

background  

Horseradish peroxidase (HRP) is a secretory plant peroxidase that catalyzes the oxidation of small aromatic substrates, such as plant hormone and lignin precursors by hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.

immunogen  

native peroxidase isolated and purified from horseradish

antibody format  

sheep

polyclonal

purified IgG in phosphate buffer saline,

lyophilized

quantity  

10 mg

for reconstitution please add 1 ml of sterile destilled water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

related products  

AS09 549 | anti-HRP horseradish (affinity purified)

additional information  

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information

recommended dilution  

1: 500 - 1: 2000 for techniques listed above

expected | apparent MW  

40 kDa

confirmed reactivity  

Armoracia rusticana

predicted reactivity  

Armoracia rusticana

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

selected references  

to be added when available





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