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product information
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| background |
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Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence. |
| immunogen |
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His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86 |
| antibody format |
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rabbit |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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200 µl, |
for reconstitution add 200 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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n.a. |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5000 with standard ECL (WB) |
| expected | apparent MW |
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16 kDA |
| confirmed reactivity |
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Arabidopsis thaliana, Spinacia oleracea |
| predicted reactivity |
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Populus spp. , monocots including Triticum aestivum, Oryza sativa, Zea mays |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions. |
| selected references |
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Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant. Petersson et al. (2006). The Prx Q protein of Arabidopsis thaliana is a member of the luminal chloroplast proteome FEBS Letters, Vol 580: 6055-6061. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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