CSD2 | Chloroplastic Cu/Zn superoxide dismutase

Product no: AS06 170

AS06 170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.maritima, B.juncea, D.sanguinalis, E.crus-galli, E.tef, I.pumila, I. walleriana, L.formosana, N.cataria, M. sativa, N.rtanjensis, O.sativa, P.Sogo Yukidian cultivar V3, P.vulgaris, P.sativum, Populus sp., S.superba, S.lycopersicum, S.tuberosum, Z.mays

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  • Product Info
  • Immunogen:

    overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 4000 (WB)
    Expected | apparent MW:

    22 | 19 kDa (Arabidopsis thaliana)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Armeria maritima, Brassica juncea, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Impatiens walleriana, Iris pumila, Liquidambar formosana, Medicago sativa,Musa acuminate, Musa paradisiaca L., Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays
    Predicted reactivity: Cucumis melo, Glycine max, Pinus pinaster, Spinacia oleracea

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples:

    Reactant: Hordeum vulgare (Barley)

    Application: Western Blotting

    Pudmed ID: 28338757

    Journal: J Exp Bot

    Figure Number: 6A

    Published Date: 2017-02-01

    First Author: Kucharewicz, W., Distelfeld, A., et al.

    Impact Factor: 6.088

    Open Publication

    Immunological analyses of the relative amounts of photosynthesis-associated proteins during senescence of primary leaves from wild-type (WT) and RNAi-W1-7 plants grown at high irradiance. Samples were prepared from wild-type plants grown for 10, 14, 21, 26, 29, and 31 das, as well as from RNAi-W1-7 plants grown for 10, 14, 21, 24, 29, 31, 34, and 36 das. The analysis was done with two biological replicates. WHIRLY1, photosynthesis-related proteins, and Cu/ZnSOD were detected by specific antibodies.

  • Additional Information
  • Additional information:

    This product can be sold containing ProClin if requested.

    Additional information (application):

    Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

  • Background
  • Background:

    Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

  • Product Citations
  • Selected references: Zhuang et al (2021). EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis. Cell Rep. 2021 Jul 13;36(2):109384. doi: 10.1016/j.celrep.2021.109384. PMID: 34260941.
    Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.
    Fesharaki-Esfahani et al. (2021) A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina,Phytochemistry,Volume 187,2021,112766,ISSN 0031-9422,https://doi.org/10.1016/j.phytochem.2021.112766.
    Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd).
    Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

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