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product information
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| background |
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Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD |
| immunogen |
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overexpressed Arabidopsis thaliana Cu/ZnSOD O78310 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization. |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD AS09 524 | anti-MnSOD | manganese superoxide dismutase collection of antibodies to plant stress proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 1000 alkaline phosphatase (WB) |
| expected | apparent MW |
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22 | 19 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
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Arabidopsis thaliana, Oryza sativa, Populus sp. |
| predicted reactivity |
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dicots inlcuding: Glycine max, Pisum sativum, monocots inlcluding: Zea mays, trees: Pinus pinaster |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form |
| selected references |
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Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant. Yu et al. (2011). Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement and starch accumulation in developing endosperm of Oryza sativa. J of Proteome Res. Nov, ahead of print Ghany et al (2008). MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis. J.Biol Chem. 283: 15932-15945 |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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