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Plant/Algal cell antibodies / Environmental stress / Heat shock


HSF1 | heat shock factor 1

Art no: AS06 178
Price: 304
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product information

background  

HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.

immunogen  

C-terminal part of HSF1 (Exons 7 to 9)

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

100 µl

for reconstitution add 100 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

additional information  

to be added when available

application information

recommended dilution  

1. 3000 with standard ECL (WB)

expected | apparent MW  

78.4 | 100 kDa

confirmed reactivity  

Chlamydomonas reinhardtii

predicted reactivity  

Chlamydomonas reinhardtii

not reactive in  

higher plants

additional information  

unspecific cross-reactions at 40-50 kDa

selected references  

Schulz-Raffelt et al. (2007).. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. The Plant Journal  52: 286-295



||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com



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