|
product information
|
| background |
|
The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins. |
| immunogen |
|
HSC70 purified from wheat germ by ATP affinity chromatography |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), ELISA (ELISA), immunoprecipitation (IP) |
| related products |
|
AS05 086 | anti-UGPase (cytoplasm marker) Collection of antibodies to heat shock proteins |
| additional information |
|
This antibody will not react with bip or CSS1. |
application information
|
| recommended dilution |
|
1 : 50 000 with alkaline phosphatase (WB) |
| expected | apparent MW |
|
70 kDa |
| confirmed reactivity |
|
Cucurbita pepo, Pisum sativum, Triticum sp. |
| predicted reactivity |
|
di and monocots |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
This antibody can inhibit protein import into organelles in vitro. |
| selected references |
|
Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242 |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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