Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) P13853

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

total IgY concentration is 32.2 µg/µl

application information

1: 1000 with standard ECL (WB)

17.6 kDa

Arabidopsis thaliana

n.a.

no confirmed exceptions from predicted reactivity known at the moment

to be added when available

to be added when available

application example

10 µg of total protein from (C) Arabidopsis thaliana WT, non-stressed, (HS) Arabidopsis thaliana WT stressed at 38ºC  were separated on  15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000 from Abcam).