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Plant/Algal cell antibodies / Environmental stress / Heat shock


HSP101 | nuclear/cytoplasmatic heat shock protein

Art no: AS08 290
Price: 304
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product information

background  

Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).

immunogen  

15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

200µl

for reconstitution add 200 µl of sterile water.

storage  

store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS07 287 | anti-HSP101 | ClpB heat shock protein, C-terminal rabbit antibody

AS08 283 | anti-HSP101 | ClpB heat shock protein, N-terminal hen antibody

AS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein rabbit antibody with reactivity to Zea mays

collection of antibodies to plant heat shock proteins

additional information  

to be added when available

application information

recommended dilution  

1: 2000 with standard ECL (WB)

expected | apparent MW  

101 kDa

confirmed reactivity  

Zea mays

predicted reactivity  

Zea mays

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

this antibody will not recognize any other cereal hsp101

selected references  

Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. Plant Physiol Dec. 8 (ahead of print).

Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633. Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.



||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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