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product information
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| background |
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Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2). |
| immunogen |
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15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS07 287 | anti-HSP101 | ClpB heat shock protein, C-terminal rabbit antibody AS08 283 | anti-HSP101 | ClpB heat shock protein, N-terminal hen antibody AS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein rabbit antibody with reactivity to Zea mays collection of antibodies to plant heat shock proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 2000 with standard ECL (WB) |
| expected | apparent MW |
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101 kDa |
| confirmed reactivity |
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Zea mays |
| predicted reactivity |
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Zea mays |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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this antibody will not recognize any other cereal hsp101 |
| selected references |
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Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. Plant Physiol Dec. 8 (ahead of print). Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633. Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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