Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) P72977

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS08 350 | anti-DnaK2 | heat shock protein 70-2 (HSP70-2)

collection of antibodies to heat shock proteins

to be added when available

application information

1: 5000 with standard ECL (WB)

16.6 kDa

Synechocystis sp. PCC 6803

Synechococcus sp., Thermosynechococcus sp.

no confirmed exceptions from predicted reactivity known in the moment

on a gradient gel Hsp16.6 runs closer to 11 kDa

to be added when available

application example

 Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis  PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000 from Abcam).